- Cloning of several DNA constructs
- Transformation into competent cells
- Screening by PCR reactions & restriction enzymes digestion
- Transfection into sf9 insect cells
- Preparation of baculovirus DNA derivatives
Work with experienced scientists
Use high quality baculovirus DNA
Consult with 30 years experiences scientists
Protein Expression
Bioinsecticide
Vaccines development
Gene delivery for tissue regeneration
Gene delivery for Cancer cells
Protein engineering
Baculovirus library
Synthetic baculovirus genomic DNA
Clone the gene of interest into the suitable baculovirus transfer vector.
($1500/ one week)
-PCR/restriction enzyme digestion will be used to clone the gene of interest into a baculovirus transfer vector.
-If necessary, the subclone vector will be confirmed using restriction enzyme digestion and DNA sequence.
-The recombinant transfer vector will be prepared for further experiment.
Generate recombinant baculovirus for target gene
($2500/ three weeks)
-Transfect the insect sf9 cells with recombinant transfer vector and baculovirus DNA.
-The recombinant virus will be screened by plaque assay.
-The recombinant virus will be amplified to a 30mL high-titer stock.
Amplification of recombinant baculovirus for titer stock. ($2500/ two weeks)
-A 250mL high-titer virus stock will be generated from a low-titer stock of a recombinant virus.
-Several rounds of amplification may be required in order to increase the recombinant virus titer stock.
Recombinant Protein Expression in sf9 cells
($2000/ one week)
-One liter of insect cell culture will be generated.
-Cell pellet will be harvested and delivered.
Recombinant Protein Purification*
($2000/ one week)
-The protein will be purified using affinity column of His-tag and GST-tag recombinant proteins.
1. Purification development is not included and protein yield is variable under the baculovirus vector expression system.
2. We offer and provide optimization for protein expression, which is based on M.O.I. and different cell lines. Optimization is optional.
3. Please call us at (619) 788-8188 for a free consultation or email us at info@sheatechinc.com for additional information
The tissue specific cDNA is prepared as described in the literature. High quality cDNA will be the one of great staring materials to making a specific baculovirus library. It is under construction and is upcoming soon.
Baculovirus genomic DNA is bio-synthesized in the test tube. It is under construction and is upcoming soon.
The baculovirus library is prepared in vitro and transfected into sf9 insect cells. The virus titer is very high and estimated higher than the literature reported. It is under construction and is upcoming soon.
We have combined a couple techniques and formed a very high titer of baculovirus library. Both new products and custom services are pre-prepared and are upcoming soon.
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