Sheatech, Inc.

Sheatech, Inc.Sheatech, Inc.Sheatech, Inc.

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(619) 788-8188

Sheatech, Inc.

Sheatech, Inc.Sheatech, Inc.Sheatech, Inc.
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Baculovirus DNA Products

SuperBAC Baculovirus DNA

High quality baculovirus DNA by homologous recombination in sf9 insect cells 

100% Recombinant Virus

Without tedious plaque assay screening 

Protein Expression

Direct virus amplification for protein expression 

Reagents for BEVS

Content

Catalogue number

Amount/price


superBAC_wt

ST001

0.5 μg/$700.00


superBAC_PDI

ST002

0.5 μg/$700.00


superBAC_cdc37

ST003

0.5 μg/$700.00


pAcYM1

STV01

10 μg/$200.00


pAcYM1_L21

STV02

10 μg/$200.00


pAc

STV03

10 μg/$200.00


pAc_L21

STV04

10 μg/$200.00


pAcmcs1

STV05

10 μg/$200.00


pAcmcs2

STV06

10 μg/$200.00


pAcYM1-polyhdedin

STV11

10 μg/$200.00


pVL-GFP-his

STV12

10 μg/$200.00

Adavntages by using superBAC baculovirus DNA

High Quality superBAC Baculovirus DNA 

Low amount 0.1 ug for one transfection in sf9 cells

Protein Expression with high virus titer in BEVS

Custom Services

Downloads

SuperBACintro (pdf)Download
SuperBAC_PDIintro (pdf)Download
SuperBAC_cdc37intro (pdf)Download
pAcYM1 (pdf)Download
pAcYM1_L21 (pdf)Download
pAc (pdf)Download
pAc_L21 (pdf)Download
pAcmcs1 (pdf)Download
pAcmcs2 (pdf)Download
BV control vector polyhedrin (pdf)Download
BV control vector GFP (pdf)Download

SuperBAC Baculovirus DNA and Related Reagents

   

 Sheatech, Inc has recently developed baculovirus genomic DNA derivatives (superBAC). There are three advantages of superBAC: high quality virus genomic DNA, 100% recombinant virus, and direct amplification for protein expression in Baculovirus Expression System (BEVS).

SuperBAC provides a positive selection for creating baculovirus recombinants in preparation for generating recombinant protein expression in insect cells. SuperBAC is derived from a wild type AcMNPV genome, containing an ORF 1629 partial deletion, which prevents non-recombinant virus from replicating in insect cells. This baculovirus DNA significantly improves on the traditional method for generating recombinant baculovirus by eliminating the tedious and time-consuming steps of plaque purification.


 SuperBAC is modified and integrated with folding protein genes, including PDI and cdc37, which are regulated by the baculovirus promoter. The folding protein genes remain in the recombinant virus genome after homologous recombination in sf9 insect cells. Recently, superBAC derivatives, superBAC_PDI and superBAC_cdc37 with single modification, have also been constructed. Other products, with double modifications in baculovirus genome are also in progress and are coming soon. 


 If superBAC does not improve the folding of your protein of interest, you may need to consider using a different specific folding protein gene in the baculovirus genome. Please feel free to give us any suggestions and discuss with our scientists. We aim to provide excellent products and services for you and we are happy to construct custom superBAC derivatives, helping your research grow and succeed.  


Find out more

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