High quality baculovirus DNA by homologous recombination in sf9 insect cells
Without tedious plaque assay screening
Direct virus amplification for protein expression
High Quality superBAC Baculovirus DNA
Low amount 0.1 ug for one transfection in sf9 cells
Protein Expression with high virus titer in BEVS
Sheatech, Inc has recently developed baculovirus genomic DNA derivatives (superBAC). There are three advantages of superBAC: high quality virus genomic DNA, 100% recombinant virus, and direct amplification for protein expression in Baculovirus Expression System (BEVS).
SuperBAC provides a positive selection for creating baculovirus recombinants in preparation for generating recombinant protein expression in insect cells. SuperBAC is derived from a wild type AcMNPV genome, containing an ORF 1629 partial deletion, which prevents non-recombinant virus from replicating in insect cells. This baculovirus DNA significantly improves on the traditional method for generating recombinant baculovirus by eliminating the tedious and time-consuming steps of plaque purification.
SuperBAC is modified and integrated with folding protein genes, including PDI and cdc37, which are regulated by the baculovirus promoter. The folding protein genes remain in the recombinant virus genome after homologous recombination in sf9 insect cells. Recently, superBAC derivatives, superBAC_PDI and superBAC_cdc37 with single modification, have also been constructed. Other products, with double modifications in baculovirus genome are also in progress and are coming soon.
If superBAC does not improve the folding of your protein of interest, you may need to consider using a different specific folding protein gene in the baculovirus genome. Please feel free to give us any suggestions and discuss with our scientists. We aim to provide excellent products and services for you and we are happy to construct custom superBAC derivatives, helping your research grow and succeed.