Work with experiences scientists
Use high quality baculovirus DNA
Consult with 30 years experiences scientists
Gene delivery for tissue formation etc.
Gene delivery for Cancer cells
Synthetic baculovirus genomic DNA
The following custom services are offered at Sheatech, Inc., please call us at (619) 788-8188 for a free consultation or email us at firstname.lastname@example.org for additional information.
Clone the gene of interest into the suitable baculovirus transfer vector.
($1500/ one week)
-PCR/restriction enzyme digestion will be used to clone the gene of interest into a baculovirus transfer vector.
-If necessary, the subclone vector will be confirmed using restriction enzyme digestion and DNA sequence.
-The recombinant transfer vector will be prepared for further experiment.
Generate the recombinant baculovirus for the target gene.
($2500/ three weeks)
-Transfect the insect sf9 cells with recombinant transfer vector and baculovirus DNA.
-The recombinant virus will be screened by plaque assay.
-The recombinant virus will be amplified to a 30mL high-titer stock.
Amplification of recombinant baculovirus for titer stock. ($2500/ two weeks)
-A 250mL high-titer virus stock will be generated from a low-titer stock of a recombinant virus.
-Several rounds of amplification may be required in order to increase the recombinant virus titer stock.
Recombinant Protein Expression in sf9 cells.
($2000/ one week)
-One liter of insect cell culture will be generated.
-Cell pellet will be harvested and delivered.
Recombinant Protein Purification*
($2000/ one week)
-The protein will be purified using affinity column of His-tag and GST-tag recombinant proteins.
1. Purification development is not included and protein yield is variable under the baculovirus vector expression system.
2. We offer and provide optimization for protein expression, which is based on M.O.I. and different cell lines. Optimization is optional.
3. Price may vary depending on project.
SuperBAC_wt Baculovirus DNA
SuperBAC_PDI Baculovirus DNA
SuperBAC_cdc37 Baculovirus DNA
Transfer Vector pAcYM1
Baculovirus Polyhedrin control
Baculovirus GFP control
The tissue specific cDNA was prepared as described in the literature. High quality cDNA will be the one of great staring materials to making a specific baculovirus library. It is under construction and is upcoming soon.
Baculovirus genomic DNA was bio-synthesized in the test tube. It is under construction and is upcoming soon.
The baculovirus library was prepared in vitro and transfected into sf9 insect cells. The virus titer was very high and estimated higher than the literature reported. It is under construction and is upcoming soon.
We have combined a couple trechniques and formed a very high titer of baculovirus library. Both new products and custom services are pre-prepared and is upcoming soon. Please don't keep it to yourself and let folks know.