BEVS Protein Expression and Applications
SDS PAGE analysis of infected sf9 cells with two MOI and time points
Our scientists at the department of R & D in Sheatech, Inc. have discovered several factors, which aid in the improvement of protein expression in the Baculovirus Expression System. Not only can high quality baculovirus virus genome DNA generate high titer recombinant virus, but the enhancer in polyhedrin promoter has also provided and has been proven to show an increase in protein expression for most recombinant proteins. Both mid log insect cell culture as well as optimization conditions of infection have been considered to produce successful virus formation and protein recovery. To aid in protein folding during protein expression in BEVS, additional help virus is often incorporated.
Recently, Sheatech research group has developed a highly quality version of baculovirus DNA, SuperBAC DNA. The SuperBAC DNA, is derived from a wild type AcMNPV genome, contains an ORF 1629 partial deletion which prevents non-recombinant virus from replicating in insect cells. In addition, SuperBAC DNA provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. The SuperBAC BV DNA kit will contain the SuperBAC DNA, Insect Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. For additional information on our recent developments on Baculovirus DNA, please click to do the research for BEVS out line and recent projects etc.
Restriction enzyme mapping analysis of superBAC baculovirus DNA