SHEATECH, INC
Gene Expression and Proteomics

Products

 

Content

Catalogue number

Amount

Link

superBAC_wt

ST001

1.0 μg

SuperBACintro.pdf
236.1 KB

superBAC_PDI

ST002

0.5 μg

SuperBAC_PDIintro.pdf
234.4 KB

superBAC_cdc37

ST003

0.5 μg

SuperBAC_cdc37intro.pdf
235.2 KB

pAcYM1

STV01

10 μg

pAcYM1.pdf
295.3 KB

pAcYM1_L21

STV02

10 μg

pAcYM1_L21.pdf
342.4 KB

pAc

STV03

10 μg

pAc.pdf
293.6 KB

pAc_L21

STV04

10 μg

pAc_L21.pdf
340.0 KB

pAcmcs1

STV05

10 μg

pAcmcs1.pdf
293.9 KB

pAcmcs2

STV06

10 μg

pAcmcs2.pdf
353.8 KB

pAcYM1-polyhdedin

STV11

10 μg

BV_control_vector_polyhedrin.pdf
229.2 KB

pVL-GFP-his

STV12

10 μg

BV_control_vector_GFP.pdf
255.0 KB

Baculovirus genomic DNA with polyhedrin

STV21

1.0 μg

Baculovirus_Control_DNA_polh.pdf
244.9 KB

Baculovirus genomic DNA with GFP-his

STV22

1.0 μg

Baculovirus_control_DNA_GFP.pdf
219.9 KB


Sheatech, Inc has recently developed baculovirus genomic DNA derivatives (superBAC). There are three advantages of superBAC: high quality virus genomic DNA, 100% recombinant virus, and direct amplification for protein expression in Baculovirus Expression System (BVES). 

SuperBAC
 provides a positive selection for creating baculovirus recombinants in preparation for generating recombinant protein expression in insect cells. SuperBAC is derived from a wild type AcMNPV genome, containing an ORF 1629 partial deletion, which prevents non-recombinant virus from replicating in insect cells. This baculovirus DNA significantly improves on the traditional method for generating recombinant baculovirus by eliminating the tedious and time-consuming steps of plaque purification.

SuperBAC is modified and integrated with folding protein genes, including PDI and cdc37, which are regulated by the baculovirus promoter. The folding protein genes remain in the recombinant virus genome after homologous recombination in sf9 insect cells.

Recently, superBAC derivatives, superBAC_PDI and superBAC_cdc37 with single modification, have also been constructed. Other products, with double modifications in baculovirus genome are also in progress and are coming soon. 

If superBAC does not improve the folding of your protein of interest, you may need to consider using a different specific folding protein gene in the baculovirus genome. Please feel free to give us any suggestions and discuss with our scientists. We aim to provide excellent products and services for you and we are happy to construct custom superBAC derivatives, helping your research grow and succeed.