SHEATECH, INC
Gene Expression and Proteomics

Phosphorylation

Protocol for Phosphorylation of Oligomers


1. Add the following in any order:           2 uL of oligomer
                                                                  5 ul of T4 Ligase 10X Buffer (10 mM of ATP)
                                                                  1 ul of T4 Kinase
                                                                  42 ul of dH2O
2. Incubate at 37°C for 30 minutes.
3. Heat at 65°C for 20 minutes to inactivate kinase and denature oligomer.
4. Cool down slowly in a tray of cold water for 15 minutes.
5. Spin for 2-3 seconds and place on ice.