SHEATECH, INC
Gene Expression and Proteomics

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SHEATECH, INC. is a newly founded biotechnology company located in San Diego, CA. Focusing on innovative research in life sciences, Sheatech has two main missions: 1) provide excellent outsourcing services for protein expression in Baculovirus Expression System (BVES) and protein purification, 2) to develop novel technologies under the Baculovirus Expression System and improve the efficiency of protein expression in BVES. We are highly experienced in producing large volume protein expression with serum free medium in BVES and have successfully contracted and worked with laboratories ranging from government and university to pharmaceutical & biotechnology companies.

Baculovirus Expression System has been recognized as one of the most versatile and powerful tool for producing mass quantities of recombinant proteins. We welcome both short term and long term projects and provide great BVES Services depending on our customers’ needs and requirements. Our quotes are reasonable and competitive. An example is listed under our Custom Services section as a reference. Without a doubt, customer satisfaction is first and foremost our top priority. Please feel free to contact us for additional information and a more detailed quote. We are willing to take a look at and undertake any challenge and/or project, which may have been unsuccessfully handled by other companies. We really appreciate your business and are looking forward to making great progresses and strides on your new and current projects.




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Current News and Development in BVES!


Our scientists at the department of R & D in Sheatech, Inc. have discovered several factors, which aid in the improvement of protein expression in the Baculovirus Expression System. Not only can high quality baculovirus virus genome DNA generate high titer recombinant virus, but the enhancer in polyhedrin promoter has also provided and has been proven to show an increase in protein expression for most recombinant proteins. Both mid log insect cell culture as well as optimization conditions of infection have been considered to produce successful virus formation and protein recovery. To aid in protein folding during protein expression in BVES, additional help virus is often incorporated.


Recently, Sheatech research group has developed a highly quality version of baculovirus DNA, SuperBAC DNA. The SuperBAC DNA, is derived from a wild type AcMNPV genome, contains an ORF 1629 partial deletion which prevents non-recombinant vi
rus from replicating in insect cells. In addition, SuperBAC DNA provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. The SuperBAC BV DNA kit will contain the SuperBAC DNA, Insect Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. For additional information on our recent developments on Baculovirus DNA, please click here.

 

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*Advantages of superBAC DNA for co-transfection* 

High quality DNA for co-transfection

No background: 100% recombinant virus
Direct amplification to increase virus titer for protein expression





Coupon Offer Here
New Product:
        superBAC_wt BACULOVIRUS DNA
Please call (619) 788-8188 or click here for further information






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Great news is coming!

 

 

This new baculovirus DNA, ezBAC, was generated and digested with the unique restriction enzyme. Furthermore, both linearized ezBAC DNA and PCR fragment were modified with T5 exo nuclease, Pfu DNA polymerase & Tag DNA ligase and formed recombinant virus genomic DNA in the microtube. This biosynthesized recombinant virus genomic DNA was generated the high titer recombinant virus in the fourth day of transfection in sf9 cells. This ezBAC baculovirus DNA has a better performance than the commercial baculovirus DNA products, including BaculoGold DNA & SuperBAC DNA etc. and it has the application of protein engineering and baculovirus library in the near future.