SHEATECH, INC
Gene Expression and Proteomics

Custom Services

Custom Services

 

The following custom services are offered at Sheatech, Inc., please call us at (619) 788-8188 for a free consultation or email us at info@sheatechinc.com for additional information.


 

Project Stage

Description

Estimated Time

Estimated Price

1.

Clone the gene of interest into the suitable baculovirus transfer vector.


-PCR/restriction enzyme digestion will be used to clone the gene of interest into a baculovirus transfer vector.
-If necessary, the subclone vector will be confirmed using restriction enzyme digestion and DNA sequence.
-The recombinant transfer vector will be prepared for further experimentation.

2 weeks

$1500

2.

Generate the recombinant baculovirus for the target gene.


-Transfect the insect cells (sf9 cells) with recombinant transfer vector and baculovirus DNA.
-The recombinant virus will be screened by plaque assay.
-The recombinant virus will be amplified to a 30mL high-titer stock.

3 weeks

$2500

3.

Amplification of recombinant baculovirus for titer stock.


-A 250mL high-titer virus stock will be generated from a low-titer stock of a recombinant virus.
-Several rounds of amplification may be required in order to increase the recombinant virus titer stock.

2 weeks

$1000

4.

Protein Expression


-One liter of insect cell culture will be generated.
-Cell pellet will be harvested and delivered.

1 week

$1000

5.

Protein Purification


-The protein will be purified using affinity column of His-tag and GST-tag recombinant proteins.

1 week

$2000

 

Total


 

9 weeks

$8000

*Please note:
-Purification development is not included and protein yield is variable under the baculovirus vector expression system.
-We offer and provide optimization for protein expression, which is based on M.O.I. and different cell lines. Optimization is optional.
-Price may vary depending on project.