SHEATECH, INC
Gene Expression and Proteomics

Baculovirus DNA Developments

Our research scientists have more than 30 years of experience on baculovirus expression systems. Recently, Sheatech research group has developed a highly quality version of baculovirus DNA, superBAC DNA. The superBAC DNA is developed and prepared in high quality DNA and confirmed with restriction enzyme digestion analysis, PCR and co-transfection etc.

 

The superBAC DNA, is derived from a wild type AcMNPV genome, contains an ORF 1629 partial deletion which prevents non-recombinant virus from replicating in insect cells. In addition, superBAC DNA provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

SuperBAC is modified and integrated with folding protein genes, including PDI and cdc37, which are regulated by the baculovirus promoter. The folding protein genes remain in the recombinant virus genome after homologous recombination in sf9 insect cells. Recently, superBAC derivatives, superBAC_PDI and superBAC_cdc37 with single modification, have also been constructed. 

 



 
The s
uperBAC BV DNA kit contains the superBAC DNA, Insect Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency.


The advantages of superBAC DNA for co-transfection:

  • High quality DNA for co-transfection.
  • No background: 100% recombinant virus.
  • Direct amplification to increase virus titer for protein expression.


The following images were obtained from analysis experiments on Sheatech’s new baculovirus genomic DNA. Baculovirus genomic DNA and linear baculovirus genomic DNA were loaded onto a TAE agarose gel. (L1=10 uL of baculovirus genomic DNA; L2= 5 ul of baculovirus genomic DNA ; L3=2 ul of baculovirus genomic DNA ; L4=10 uL of linear baculovirus genomic DNA; L5=5 uL of linear baculovirus genomic DNA ; L6=2 uL of linear baculovirus genomic DNA; L7=DNA markers.) The result shows high quality virus DNA, which was prepared by our scientists in Sheatech.

 



 

Restriction enzyme digested baculovirus DNA separated in 0.6% TAE agarose gel.